stedycon sted microscope  (abberior instruments)

Journal: International Journal of Molecular Sciences

Article Title: Imaging-Based Molecular Interaction Between Src and Lamin A/C Mechanosensitive Proteins in the Nucleus of Laminopathic Cells

doi: 10.3390/ijms252413365

Figure Lengend Snippet: STED nanoscopy of Src and lamin A/C in nuclei from healthy and laminopathic fibroblasts. ( A ) In the healthy control nuclei, Src labeling (green) was thickened at the nuclear periphery, with a diffuse and dotted distribution in the nuclear matrix, whereas some anomalous aggregates were observed (arrows) in patients’ nuclei. Alterations in the structural organization of the lamin A/C (red) meshworks have been seen in several nuclei of the fibroblasts of patient 1 and patient 2 (arrowheads). Colocalization masks (yellow) showed the co-distribution of Src and lamin A/C at the nuclear rim in all samples and a higher concentration in the nucleoplasm of patients’ cells (high magnification of insets). Bars: 5 µm and 2 µm. ( B ) Mean values of the overlap coefficient quantified in STED images of double-stained fibroblast nuclei. (** p < 0.01; * p < 0.05).

Article Snippet: STED imaging was performed on a STED microscope platform (STEDYCON, Abberior Instruments GmbH, Göttingen, Germany) equipped with an upright Zeiss Axioimager Z.2 microscope (Zeiss Microscopy GmbH, Oberkochen, Germany) and a pulsed STED laser (Abberior Instruments GmbH) with a depletion wavelength of 775 nm working at a repetition rate of 40 MHz, a pulse duration of 1 ns, using STEDYCON SmartControl software version 9.

Techniques: Control, Labeling, Concentration Assay, Staining